This article conducts a comparative study on the microstructure and physical and mechanical properties of Lanzhou ANBR3305 and NBR3305E, including tests on micro-condensed structure such as glass transition temperature, acrylonitrile content, and content of various sequence structures, as well as tests on the processing properties, vulcanization characteristics, physical and mechanical properties, and heat-resistant oxygen aging resistance of vulcanized rubber. The results indicate that ANBR3305 exhibits superior heat-resistant oxygen aging resistance compared to NBR3305E when no antioxidant is added, and when antioxidants 4010NA, RD and BLE are added; antioxidant NAPM can significantly improve the heat-resistant oxygen aging resistance of NBR3305E; 4010NA and RD are beneficial to improving the heat-resistant oxygen aging resistance of ANBR3305; BLE is detrimental to its heat-resistant oxygen aging resistance.